Modify Your NSC 14613SKI II Into A Complete Goldmine

m strains Genin and Boucher suggested that the presence of small plasmids in R. solanacearum cells, initially described by Morales and Sequeira, was more an exception than a rule. Ferrostatin-1 However, we found small plasmids in the African and Indonesian strains. These plasmids were named pRSC35 in strain CMR15 and pRSI13 in strain PSI07. The presence of small plasmids is therefore maybe less rare in R. solanacearum strains than previously thought. These small plasmids may have remained unde tected until now because their very low copy number makes them difficult to purify, Despite their low copy numbers, the stability of these plasmids is apparently ensured by two different toxin antitoxin systems.
On pRSC35, two CDS had a lim ited homology with zeta toxin and epsilon antitoxin, which form a post segregational mechanism for plasmid NSC 14613 maintenance in bacteria, The regulator was not detected in the CMR15 genome. This zeta epsi lon TA system is well described and a similar system con fers a bactericidal effect on Bacillus subtilis, and bacteriostatic effects on E. coli, The plasmid SKI II pRSC35 was broadly syntenic with plas mids from many plant associated bacteria including pXcB of Xanthomonas citri pv. aurantifolii, diverse P. putida plasmids, a X. citri pv. citri plasmid and a plasmid from X. euvesicatoria, Among the 44 CDS present on this plasmid, 14 appeared to be involved in the Type IV secretion system. 10 genes make up the virB operon ranging from 5 to 15 kbp, and four genes form the tra operon from 28 to 34 kbp.
Eight CDS coded for proteins potentially involved in DNA metabo lism, Finaly, one CDS had a strong homology to a Zn metalloprotease, Ribonucleotide also carried on plasmids in several human and or animal pathogenic bacteria or opportunis tic bacteria. P. putida, Yersina pestis, Escherichia coli O157. H7, Klebsiella pneumoniae, Salmonella enterica, etc. Metalloproteases AZD3514 like those encoded on pRSC35 are essential for the infection process of many eukaryotes, Ferrostatin-1 The unexpected Type IV Secretion System is unique among R. solanacearum strains studied to date and could play diverse important roles in virulence and adaptation. The CMR15 Type IV secretion system genes, which are clustered together with the virB operon, have nearly the same organization as on pXAC64 of Xanthomonas citri pv citri, The type IV secretion system is a bacterial conjugation apparatus and the DNA thus efficiently imported through the cell envelope can directly increase the fitness or virulence of bacteria by mediating the acquisition of new traits like effectors or antibiotic resis tance genes.
AZD3514 Type IV secretion systems can also be directly involved in virulence via direct injection of effec tors or DNA into plant cells, No obvious type IV effectors were found on pRSC35 or in the complete genome of CMR15, but some proteins of unknown func tion could be Type IV effectors. Additional experiments are needed to investigate the distribution of this plas mid in African phylotype Ferrostatin-1 III strains, the ecological and pathogenic role of this plasmid in the phenotype of phylotype III strain CMR15, and the occurrence of such plasmids in strains belonging to other phylotypes.
A second low copy number plasmid, pRSI13, was pres ent in PSI07. It was syntenic with a plasmid found in Nitrobacter hamburgensis X14, Burkholderia pseudomallei AZD3514 9 and 91, Parvibaculum lavamentivorans DS 1, Aci dovorax sp. JS42 and E. coli pOLA52, pRSI13 contained 23 CDS, 16 of which encoded for proteins of unknown function and one for a putative transcriptional regulator. Other pRSI13 CDS coded for proteins puta tively involved in DNA metabolism or conjugation, Thus, the functional annotation reveals no obvious role for this plasmid in either the ecology of the bacteria or during pathogenesis. The maintenance of this plasmid seems likely due to the TA system rather than to increased fitness. New insight into the phylogeny of the R. solanacearum species complex Genomes were compared pairwise using the average nucleotide identity